Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 133, Issue -, Pages 656-662Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.03.218
Keywords
Sargassum fusiforme; Fucoidan; P-selectin
Funding
- National Natural Science Foundation of China [41876197, 31470430, 81872952]
- Natural Science Foundation of Zhejiang Province [LY18C020006, LGN18C020004]
- Science and Technology Program of Wenzhou [Y20180210]
- Scientific Foundation of Education Department of Zhejiang Province [Y201737374]
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P-selectin, mediated adhesion between endothelium and neutrophils, is a promising target for the therapeutics of acute inflammatory-related diseases. It is reported that brown algal fucoidans can antagonize P-selectin function. However, the fractionation and physicochemical characterization of Sargassum fusiforme fucoidan, and the screening of fucoidan fractions with P-selectin antagonistic capability have not been investigated. In this study, we isolated and fractionated systematically the S. fusiforme fucoidan by ion-exchange chromatography and size exclusion chromatography to obtain eight fucoidan fractions. Their physicochemical characterization was determined by chemical methods, HPLC and FT-IR. The inhibitory capacity of the fucoidan fractions in P-selectin-mediated leukocyte adhesion was evaluated by static adhesion assay and parallel-plate flow chamber. Results showed that fucoidan fractions possessed distinct physicochemical properties, including total carbohydrate, uronic acid and sulfate contents, molecular weight, and monosaccharide compositions. Among all the fucoidan fractions, SFF-32 and SFF-42 showed better blocking ability against P-selectin-mediated cell adhesion. (C) 2019 Elsevier B.V. All rights reserved.
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