Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 122, Issue -, Pages 320-328Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.10.182
Keywords
Carrageenan hydrogel; Dimethyloxallylglycine; Whitlockite nanoparticles; Angiogenesis; Osteogenesis
Funding
- Department of Science and Technology (DST), India [DST/INT/Korea/P-41, NRF-2017K1A3A1A19070731]
- National Research Foundation of Korea (NRF), Korea [DST/INT/Korea/P-41, NRF-2017K1A3A1A19070731]
- Nanomission, Department of Science and Technology (DST), Government of India under the the 'M.Tech' program [SR/NM/PG-01/2015]
- Council of Scientific and Industrial Research (CSIR) [09/963 (0038) 2K17-EMR-I]
- National Research Foundation of Korea [2017K1A3A1A19070731] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Functional biomaterials that couple angiogenesis and osteogenesis processes are vital for bone tissue engineering and bone remodeling. Herein we developed an injectable carrageenan nanocomposite hydrogel incorporated with whitlockite nanoparticles and an angiogenic drug, dimethyloxallylglycine. Synthesized whitlockite nano particles and nanocomposite hydrogels were characterized using SEM, TEM, EDS and MR Developed hydrogels were injectable, mechanically stable, cytocompatible and has better protein adsorption. Incorporation of dimethyloxallylglycine resulted in initial burst release followed by sustained release for 7 days. Human umbilical vein endothelial cells exposed to dimethyloxallylglycine incorporated nanocomposite hydrogel showed enhanced cell migration and capillary tube-like structure formation. Osteogenic differentiation in rat adipose derived mesenchymal stem cells after 7 and 14 days revealed increased levels of alkaline phosphatase activity in vitro. Furthermore, cells exposed to nanocomposite hydrogel revealed enhanced protein expressions of RUNX2, COL and OPN. Overall, these results suggest that incorporation of whitlockite and dimethyloxallylglycine in carrageenan hydrogel promoted osteogenesis and angiogenesis in vitro. (C) 2018 Elsevier B.V. All rights reserved.
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