Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 68, Issue -, Pages 234-241Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2018.12.042
Keywords
Ischemic reperfusion; NLRP3 inflammasome; Autophagy; GSK-3 beta
Categories
Funding
- National Natural Science Foundation of China [81671158, 81771261]
- National Youth Foundation of China [81701165]
- Natural Science Foundation of Chongqing Science and Technology Committee, China [cstc2015jcyjA10048]
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The nod-like receptor protein 3 (NLRP3) inflammasome has a critical role in cerebral ischemic injury, and autophagy is related to activation of the inflammasome under oxidative stress conditions. However, it is unclear how NLRP3 inflammasome activation is regulated. Glycogen synthase kinase 3 beta (GSK-3 beta) emerged as an important risk factor for brain ischemia reperfusion injury, and GSK-3 beta inhibits autophagic activity in many diseases. In this study, we examined whether NLRP3 inflammasome-derived inflammation could be ameliorated by GSK-313 inhibition in a cerebral ischemia reperfusion injury model and assessed whether autophagy is involved in this process. To establish ischemic reperfusion injury, we used a middle cerebral artery occlusion-reperfusion (MCAO/R) model in rats. A chemical inhibitor (SB216763) and GSK-3 beta siRNA were used to suppress GSK-3 beta activation and GSK-3 beta expression in vivo. The results demonstrated that SB216763 and GSK-30 siRNA improved neurological scores, reduced cerebral infarct volume, and decreased the levels of NLRP3 inflammasome, cleavedcaspase-1, IL-4, and IL-18. Inhibiting GSK-3 beta activation enhanced autophagic activity (ratio of LC3B-II/LC3B-I and p62/SQSTM1), whereas treating with an autophagy inhibitor (3-MA) abrogated the inhibitory effect on NLRP3 inflammasome activation after GSK-3 beta inhibition. These results suggest that inhibiting GSK-3 beta down-regulates NLRP3 inflammasome expression by increasing autophagic activity in cerebral ischemia reperfusion injury. GSK-3 beta might be an attractive specific target and that it functions by regulating the NLRP3 inflammasome.
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