4.2 Article

Synergistic evaluation of AgO2 nanoparticles with ceftriaxone against CTXM and blaSHV genes positive ESBL producing clinical strains of Uro-pathogenic E. coli

Journal

IET NANOBIOTECHNOLOGY
Volume 13, Issue 4, Pages 435-440

Publisher

WILEY
DOI: 10.1049/iet-nbt.2018.5415

Keywords

antibacterial activity; nanofabrication; nanomedicine; drugs; nanoparticles; microorganisms; crystallites; scanning electron microscopy; silver compounds; X-ray diffraction; X-ray chemical analysis; Fourier transform infrared spectra; organic compounds; genetics; synergistic evaluation; clinical strains; silver oxide nanoparticles; silver foil; wet chemical method; X-ray diffraction analysis; crystallographic structures; standard cubic structure; spherical-shaped nanoparticles; energy dispersive X-ray analysis; ceftriaxone drug; synergistic activity; ESBL producer; scanning electron microscopy; Fourier transform infrared analysis; Escherichia coli; blaSHV gene positive ESBL; crystallite size; random distribution; lactam antibiotics; MDR-ESBL E; coli strains; polymorphism; blaCTXM; uro-pathogenic infections; uro-pathogenic E; coli; AgO2

Funding

  1. International University Islamabad, Pakistan Institute of Engineering and Applied Sciences, Institute of Biomedical and Genetic Engineering Islamabad and Higher Education Commission of Pakistan

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The silver oxide nanoparticles (AgO2-NPs) were synthesised using silver foil as a new precursor in wet chemical method. X-ray diffraction analysis shows crystallographic structures of AgO2-NPs with crystallite size of 35.54 nm well-matched with standard cubic structure. Scanning electron microscopy analysis clearly shows the random distribution of spherical-shaped nanoparticles. Energy dispersive X-ray analysis confirmed the purity of the samples as it shows no impurity element. Fourier transforms infra-red analysis confirmed the formation of AgO2-NPs with the presence of Ag-O-Ag stretching bond. All the techniques also confirmed the loading of ceftriaxone drug on the surface of AgO2-NPs. This study also described the effect of AgO2-NPs having synergistic activity with beta lactam antibiotic i.e. ceftriaxone against ESBL generating Escherichia coli (E. coli). Among isolated strains of E. coli, 60.0% were found to be ESBL producer. The synergistic activities of AgO2-NPs with ceftriaxone suggest that these combinations are effective against MDR-ESBL E. coli strains as evident by increase in zone sizes. The present study observed rise in MDR-ESBL E. coli with polymorphism of blaCTXM and blaSHV causing UTI infections in Pakistani population. The antibiotic and AgO2-NPs synergistic effect can be used as an efficient approach to combat uro-pathogenic infections.

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