Journal
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Volume 55, Issue 6, Pages 889-898Publisher
AMER THORACIC SOC
DOI: 10.1165/rcmb.2015-0237OC
Keywords
hypoxia; complement regulatory proteins; lung
Funding
- National Institutes of Health (NIH) [HL067177, HL096845, P01AI084853]
- NIH [T32 AI060519]
- MRC [G1001367] Funding Source: UKRI
- Medical Research Council [G1001367] Funding Source: researchfish
Ask authors/readers for more resources
Airway epithelial CD55 down-regulation occurs in several hypoxia-associated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxia-inducible factor (HIF)-1 alpha in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on small-airway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1 alpha during hypoxia-recovered CD55 expression in small-airway epithelial cells. HIF-1 alpha overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1 alpha in regulating the expression of CD55 on airway epithelium.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available