Journal
FOOD CHEMISTRY
Volume 290, Issue -, Pages 229-238Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.03.145
Keywords
Folin-Ciocalteu; High-performance liquid chromatography; Bioactive phenolics; Oxygen radical absorbance capacity; Ferric reducing antioxidant power; RAW 264.7 macrophages; Cytotoxicity; NF-kappa B activation
Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [88887.169471/2018-00]
- Fondo Nacional de Desarrollo Cientifico y Tecnologico - FONDECYT postdoctorado [3180432]
- Fundacion para la Innovacion Agraria -FIA [PYT-2017-0490]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [303188/2016-2]
- FAPESP [2008/55492-7, 2016/15563-9]
- CNPq [310522/2015-3]
- Natural Science and Engineering Research Council (NSERC) of Canada
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [16/15563-9] Funding Source: FAPESP
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Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-kappa 3 using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-kappa B. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-kappa 3 (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application.
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