Journal
FOOD CHEMISTRY
Volume 277, Issue -, Pages 273-278Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.10.130
Keywords
Fluorescence; Ochratoxin A; RNase H; Signal amplification
Funding
- National Natural Science Foundation of China [21205142, 31370104]
- State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [2017006]
- Research Innovation Program for Graduates of Central South University [2016zzts580, 2017zzts347]
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A new assay for the selective and sensitive aptamer-based fluorescent biosensor for ochratoxin A (OTA) detection is proposed. It consists of a ribonuclease H (RNase H)-assisted cycle response that leads to a significant amplification of the signal, which allow for a low limit of detection (LOD) of 0.08 ng/mL. By using this technique, a high selectivity for OTA against ochratoxin B and aflatoxin B-1 (AFB(1)) was noticed. The quantitative determination in real samples has been verified using red wine samples spiked with a series of OTA concentrations (0.4, 4, and 12 ng/mL), and the recoveries ranged from 96.1 to 107.5%. This aptasensor has great practical applications in food industry and moreover, can be extended for the detection of other toxins by replacing the sequence of the recognition aptamer.
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