Journal
FOOD ANALYTICAL METHODS
Volume 12, Issue 5, Pages 1255-1261Publisher
SPRINGER
DOI: 10.1007/s12161-019-01465-z
Keywords
Melamine; G-quadruplex; Rolling circle amplification; Fluorescence; Signal amplification
Categories
Funding
- National Natural Science Foundation of China [21505010, 21675128]
Ask authors/readers for more resources
Sensitive identification and detection of melamine illegally added to food to increase false protein content is important for food safety monitoring because melamine can cause tissue injury or even death above the safety regulation level. Melamine can coordinate with two thymines to form stable T-melamine-T structures. On the basis of such a unique interaction, we have developed a T-melamine-T base mismatch-mediated rolling circle amplification (RCA) signal enhancement means for amplified sensitive and label-free fluorescent detection of melamine in milk. The presence of melamine facilitates the hybridization between two T:T base mismatch containing DNA strands, which triggers RCA generation of many long sequences with repeated G-quadruplex segments. The fluorescent dye, thioflavin T, further binds the resulting G-quadruplexes to exhibit substantially enhanced fluorescence for sensitive detection of melamine at the low nanomolar level in a label-free manner. Besides, the developed approach is highly selective toward the discrimination and detection of melamine in milk samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available