Automatically High-Throughput Quantification by Paper Spray Ionization Mass Spectrometry for Multiple Pesticides in Wine

A rapid paper spray (PS) ionization tandem mass spectrometry analytical method has been developed to determine multiple pesticides in red wine. Without chromatographic separation, 36 pesticides in wine could be simultaneously determined less than 9 and 3min, followed by off-line QuEChERS treatment and on-line paper adsorption treatment, respectively. Isotopically labeled internal standard (IS) was utilized to calibrate injection process, and linear ranges were one to three orders of magnitude for different pesticides. LODs were ranging between 0.1 and 100ng/mL, and LOQs were ranging between 0.3 and 300ng/mL. Method validation was used to evaluate the accuracy and precision, and the results for most analytes were at the range of 75-120%. All the data processes were automatically achieved by build-in instrument software. These methods were also compared with QuEChERS-DART-MS/MS and DART-MS/MS. The results demonstrated that PS-MS/MS was a rapid and simple method to quantify multiple pesticides in red wine.