Journal
FISHERIES SCIENCE
Volume 85, Issue 3, Pages 591-599Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s12562-019-01303-5
Keywords
Alcalase; Collagen; Papain; Skin; Yellowfin tuna
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Funding
- Indonesian Ministry of Research, Technology, and Higher Education through competitive funding in joint research and publication (KLN)
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Collagen is one of the biopolymer proteins that may be useful for food and non-food purposes. Recently, even for collagen, it is becoming necessary to meet the requirements of halal for Muslim societies, and thus fish-derived collagen is regarded as ideal. Utilization of tuna skin as an alternative source of collagen would increase its market value, and there is an abundant catch in south west Asian regions. Thus, the purpose of this study was to prepare and characterize collagen from the skin of yellowfin tuna using a plant-derived protease, papain. The obtained collagen was further hydrolyzed with Alcalase, and the peptides fractionated by gel filtration chromatography were subjected to antioxidant and antiglycation activity assays. The results showed high activities of hydrolysate in these parameters.
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