Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 53, Issue 4, Pages 2141-2150Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.8b05907
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Funding
- National Natural Science Foundation of China [51608253, 51678290, 51438008]
- Natural Science Foundation of Jiangsu Province, China [BK20160656]
- Fundamental Research Funds for the Central Universities
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Disinfection regimes are considered the most solid strategy to reduce microbial risks in drinking water, but their roles in shaping the antibiotic resistome are poorly understood. This study revealed the alteration of antibiotic resistance genes (ARGs) profiles, their co-occurrence with mobile genetic elements (MGEs), and potential hosts during drinking water disinfection based on metagenomic assembly. We found the ozone/chlorine (O-3/Cl-2) coupled disinfection significantly increased the relative abundance of ARGs and MGE-carrying antibiotic resistance contigs (ARCs) through the enrichment of ARGs within the resistance-nodulation-cell-division and ATP-binding cassette antibiotic efflux families that are primarily carried by Pseudomonas, Acinetobacter, Mycobacterium, and Methylocystis, whereas the antimicrobial resin/chlorine coupled disinfection posed unremarkable changes to the ARG and MGE abundances. Moreover, the co-occurrence patterns of antibiotic efflux and beta-lactam ARGs and MGEs were widely identified, and ARCs carrying the recR and mexH genes were detected in all the samples, with the highest abundance of 2.25 X 10(-2) copies per cell after O-3/Cl-2 disinfection. Sequence-independent binning analysis successfully retrieved two draft ARG-carrying genomes of Acidovorax sp. MR-S7 and Hydrogenophaga sp. IBVHS2 further revealing the host-ARG relationship during O-3/Cl-2 disinfection. Overall, this study provides novel insights into the antibiotic resistome alteration during drinking water disinfection.
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