Journal
ELECTROCHIMICA ACTA
Volume 295, Issue -, Pages 615-623Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2018.10.177
Keywords
Porous graphene; Catalase; H2O2; 2,4-D; Inhibition
Categories
Funding
- NSFC [21675140, 21575124, 21275124, 21275125, 21465004]
- Foundation of Yangzhou Environmental Protection Bureau [YHK1410]
- high-end talent project of Yangzhou University
- 14th Six talent peaks project in Jiangsu Province [SWYY-085]
- Higher Education Outstanding Scientific and Technological Innovation Team of Jiangsu Province [2017-6]
- Young academic leaders of Jiangsu Province - PAPD
- Guangxi Youth Scientific Fund [2017KY0832]
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This work presented a facile and green method to prepare porous graphene (PGR) using a combination of sodium citrate treatment, hydrothermal reduction and lyophilization. The structure of the obtained PGR was analyzed by X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), Brunauer-Emmett-Teller (BET), and the morphology was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Furthermore, this PGR was used as the support for the immobilization of catalase. Fourier-transform infrared spectroscopy (FT-IR) spectra indicated that the immobilized catalase kept its structure without any change. Electrochemical impedance spectroscopy and cyclic voltammetry indicated the fast electron transfer between the immobilized catalase and the glassy carbon electrode (GCE). This biosensor showed a linearity in the 1.0 x 10(-7) - 7.7 x 10(-6) mol L-1 concentration range with a low detection limit of 83.0 nmol L-1 (3 delta/S) for H2O2. In addition, the biosensor was also applied to measure 2,4-dichlorophenoxyacetic acid (2,4-D) concentration based on catalase enzymatic inhibition. Under the optimized conditions, two linear relationships of the inhibition ratios vs. the 2,4-D concentrations ranging from 2.4 x 10(-9) similar to 2.6 x 10(-8) mol L-1 and 2.6 x 10(-8) similar to 3.6 x 10(-7) mol L-1 were found. The detection limit for 2,4-D was down to 1.5 nmol L-1 at 3 delta/S. The inhibition constants (K-i) of 2,4-D to the immobilized catalase was calculated to be 6.50 x 10(-8) mol L-1. This biosensor was applied for the determination of H2O2 and 2,4-D in real samples with satisfactory results. (C) 2018 Elsevier Ltd. All rights reserved.
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