Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 94, Issue 4, Pages 321-325Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2019.03.003
Keywords
Cefiderocol; Siderophore; MIC; Reproducibility; Iron-depleted; Cation-adjusted Mueller-Hinton broth; Gram-negative bacilli
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Funding
- Shionogi & Co., Ltd., Osaka, Japan
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In 2017, the Clinical and Laboratory Standards Institute (CLSI) Subcommittee on Antimicrobial Susceptibility Testing approved the use of iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) prepared with Chelex (R) 100 resin (Bio-Rad Laboratories, Hercules, CA) to determine MICs for cefiderocol. The current study examined the reproducibility of cefiderocol MICs generated for 19 clinical isolates of Gram-negative bacilli, with CAMHB produced by three manufacturers; each of the 19 isolates was tested for 10 replicates in ID-CAMHB from each manufacturer. When analyzed by individual media lot, greater than 95% of MIC results were within +/- one doubling-dilution of the mode for each of the 19 isolates tested. The remaining 5.0% of MIC results were within +/- two doubling-dilutions of the modal MIC. For all media lots combined, 92.2% of MIC results were within +/- one doubling-dilution of the modal MIC for each isolate, 99.8% were within +/- two doubling-dilutions and 100% were within three doubling-dilutions. (C) 2019 The Authors. Published by Elsevier Inc.
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