Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 175, Issue -, Pages 614-624Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2018.12.029
Keywords
Biodegradable; Carboxymethxyl; Chitin; Enzymatically catalyzed; Injectable hydrogel; Tissue engineering
Funding
- National Natural Science Foundation of China [21674083, 21875168, 51533006]
- Natural Science Foundation of Jiangsu Province of China [BK20151249]
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The in-situ forming injectable hydrogels have received much attention as scaffolds in the biomedical field, providing a minimally invasive surgical procedure to fill the damaged area. In the present work, carboxymethyl chitin (CMCH) synthesized homogenously was further functionalized with tyramine, resulted in a new injectable enzymatically crosslinked in-situ forming hydrogel under physiological conditions. This new tyramine-modified carboxymethyl chitin (CMCH-Tyr) hydrogel showed much better mechanical properties than those of the thermosensitive in-situ forming physical-crosslinking CMCH hydrogel. The CMCH-Tyr hydrogels remained stable under physiological conditions and could be degraded by lysozyme. The gelation time, strength and biodegradation rate of the CMCH-Tyr hydrogels can be adjusted by varying the concentrations of the horseradish peroxidase and H2O2 in the certain range. In vitro cytotoxicity assays and in vivo in-situ injection study showed non-toxicity, favorable gel formation, and good tissue biocompatibility of the enzyme-catalyzed CMCH-Tyr hydrogel. Thus, the biodegradable and biocompatible CMCH-Tyr hydrogels may hold great potential for three dimensional cell culture and tissue engineering.
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