Journal
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
Volume 14, Issue 3, Pages 364-377Publisher
AMER SOC NEPHROLOGY
DOI: 10.2215/CJN.05830518
Keywords
Complement; hemolytic uremic syndrome; pathogenic variants; Atypical Hemolytic Uremic Syndrome; complement factor H; human; Complement Factor H; Shiga Toxin; CD46 protein; human; Membrane Cofactor Protein; Escherichia coli; Genetic Background; High-Throughput Nucleotide Sequencing; Complement System Proteins; Complement Activation; Kidney Failure, Chronic; Genotype; Gene Frequency; Biomarkers
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Funding
- Programme Hospitalier de Recherche Clinique [AOR 09 077, AOM08198]
- European Union FP7 grant [2012-305608]
- Fondation du rein (FRM Prix 2012 FDR)
- Association pour l'Information et la Recherche dans les maladies Renales genetiques (AIRG France)
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Background and objectives Inherited complement hyperactivation is critical for the pathogenesis of atypical hemolytic uremic syndrome (HUS) but undetermined in postdiarrheal HUS. Our aim was to investigate complement activation and variants of complement genes, and their associationwith disease severity in children with Shiga toxin-associated HUS. Design, setting, participants, & measurements Determination of complement biomarkers levels and next-generation sequencing for the six susceptibility genes for atypical HUS were performed in 108 children with a clinical diagnosis of post-diarrheal HUS (75 Shiga toxin-positive, and 33 Shiga toxin-negative) and 80 French controls. As an independent control cohort, we analyzed the genotypes in 503 European individuals fromthe 1000 Genomes Project. Results During the acute phase of HUS, plasma levels of C3 and sC5b-9 were increased, and half of patients had decreased membrane cofactor protein expression, which normalized after 2 weeks. Variants with minor allele frequency,1% were identified in 12 Shiga toxin-positive patients with HUS (12 out of 75, 16%), including pathogenic variants in four (four out of 75, 5%), with no significant differences compared with Shiga toxin-negative patients with HUS and controls. Pathogenic variants with minor allele frequency,0.1% were found in three Shiga toxin-positive patientswithHUS (three out of 75, 4%) versus only four European controls (four out of 503, 0.8%) (odds ratio, 5.2; 95% confidence interval, 1.1 to 24; P= 0.03). The genetic background didnot significantly affect dialysis requirement, neurologic manifestations, and sC5b-9 level during the acute phase, and incident CKD during follow-up. However, the only patient who progressed to ESKD within 3 years carried a factor H pathogenic variant. Conclusions Rare variants and complement activation biomarkers were not associated with severity of Shiga toxin-associated HUS. Only pathogenic variants with minor allele frequency,0.1% are more frequent in Shiga toxin-positive patients with HUS than in controls.
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