A ratiometric fluorescent probe for the detection of hypochlorous acid in living cells and zebra fish with a long wavelength emission

A ratiometric fluorescence probe, NClO, for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative. Probe NClO displayed a red emission (lambda(max) = 615 nm). In the presence of HClO, the solution of probe NClO gave off a strong green fluorescence (lambda(em.) (max) = 520 nm) with a rapid response (within seconds). This probe had been applied to image HClO in living cells and zebra fish. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.