Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 14, Issue 6, Pages 904-909Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201900150
Keywords
aggregation-induced emission; cell imaging; fluorescent probes; tetraphenylethene; unfolded proteins
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Funding
- Australian Research Council [DE170100058]
- Rebecca L. Cooper Medical Research Foundation [PG2018043]
- Australian Research Council (ARC) [FT130100500]
- ARC Centre of Excellence in Exciton Science [CE170100026]
- Australian Research Council [FT130100500, DE170100058] Funding Source: Australian Research Council
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Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation of unfolded proteins, which has been found to associate with a number of disease conditions including neurodegenerative diseases, diabetes and inflammation. Here we report a maleimide-functionalized tetraphenylethene (TPE)-derivatized fluorescent dye, TPE-NMI, which shows fluorescence turn-on property upon reacting with unfolded proteins in vitro and in live cells under proteostatic stress conditions. The level of unfolded proteins can be measured by flow cytometry and visualized with confocal microscopy.
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