Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 186, Issue 4, Pages 1044-1054Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2015.11.019
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Funding
- Landelijke Stichting voor Blinden en Slechtzienden
- Stichting Oogfonds Nederland
- Stichting Winckel Sweep
- UitZicht grant
- Nederlandse Vereniging ter Verbetering van het Lot der Blinden
- Rotterdamse Stichting Blindenbelangen grant [B20120030]
- Stichting Blindenhulp
- Stichting Nederlands Oogheelkundig Onderzoek grant [2012-05]
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Loss of blood-retinal barrier (BRB) properties induced by vascular endothelial growth factor (VEGF) and other factors is an important cause of diabetic macular edema. Previously, we found that the presence of plasmalemma vesicle associated protein (PLVAP) in retinal capillaries associates with loss of BRB properties and correlates with increased vascular permeability in diabetic macular edema. In this study, we investigated whether absence of PLVAP protects the BRB from VEGF-induced permeability. We used lentiviral-delivered shRNA or siRNA to inhibit PLVAP expression. The barrier properties of in vitro BRB models were assessed by measuring transendothelial electrical resistance, permeability of differently sized tracers, and the presence of endothelial junction complexes. The effect of VEGF on caveolae formation was studied in human retinal explants. BRB loss in vivo was studied in the mouse oxygen-induced retinopathy model. The inhibition of PLVAP expression resulted in decreased VEGF-induced BRB permeability of fluorescent tracers, both in vivo and in vitro. PLVAP inhibition attenuated transendothelial electrical resistance reduction induced by VEGF in BRB models in vitro and significantly increased transendothelial electrical resistance of the nonbarrier human umbilical vein endothelial cells. Furthermore, PLVAP knockdown prevented VEGF-induced caveolae formation in retinal explants but did not rescue VEGF-induced alterations in endothelial junction complexes. In conclusion, PLVAP is an essential cofactor in VEGF-induced BRB permeability and may become an interesting novel target for diabetic macular edema therapy.
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