Journal
CHEMCATCHEM
Volume 11, Issue 16, Pages 4171-4181Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cctc.201900333
Keywords
carboxylate reductase (CAR); in vivo biotransformation; carboxylic acid; reduction; antioxidant
Categories
Funding
- Austrian Research Promotion Agency FFG [P 860262]
- Firmenich SA
- Austrian BMWFW
- BMVIT
- SFG
- Standort Tirol
- Government of Lower Austria
- ZIT through Austrian FFG-COMET-Funding Program
Ask authors/readers for more resources
Fragrance and flavor industries could not imagine business without aldehydes. Processes for their commercial production raise environmental and ecological concerns. The chemical reduction of organic acids to aldehydes is challenging. To fulfill the demand of a mild and selective reduction of carboxylic acids to aldehydes, carboxylic acid reductases (CARs) are gaining importance. We identified two new subtype IV fungal CARs from Dichomitus squalens CAR (DsCAR) and Trametes versicolor CAR (Tv2CAR) in addition to literature known Trametes versicolor CAR (TvCAR). Expression levels were improved by the co-expression of GroEL-GroES with either the trigger factor or the DnaJ-DnaK-GrpE system. Investigation of the substrate scope of the three enzymes revealed overlapping substrate specificities. Tv2CAR and DsCAR showed a preferred pH range of 7.0 to 8.0 in bicine buffer. TvCAR showed highest activity at pH 6.5 to 7.5 in MES buffer and slightly reduced activity at pH 6.0 or 8.0. TvCAR appeared to tolerate a wider pH range without significant loss of activity. Type IV fungal CARs optimal temperature was in the range of 25-35 degrees C. TvCAR showed a melting temperature (T-m) of 55 degrees C indicating higher stability compared to type III and the other type IV fungal CARs (T-m 51-52 degrees C). Finally, TvCAR was used as the key enzyme for the bioreduction of 3,4-dihydroxyphenylacetic acid to the antioxidant 3-hydroxytyrosol (3-HT) and gave 58 mM of 3-HT after 24 h, which correlates to a productivity of 0.37 gL(-1) h(-1).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available