Journal
CELL STRESS & CHAPERONES
Volume 24, Issue 3, Pages 517-525Publisher
SPRINGER
DOI: 10.1007/s12192-019-00980-6
Keywords
Portunus trituberculatus; Crustacean hyperglycemic hormone; Alternative splicing; Osmoregulation
Categories
Funding
- National Natural Science Foundation of China [41776160, 41576147]
- Efficient Eco Agriculture Innovation Project of Taishan Leading Talent Project [LJNY2015002]
- Shandong Province Key Development Program for Research [2018GSF121030]
- Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology [2016LMFSA12]
- Qingdao Applied Basic Research Project [17-1-1-95-jch]
- Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institute [20603022018027]
- Key Research and Development Plan of Jiangsu Province [BE2017325]
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The crustacean hyperglycemic hormone (CHH) gene of Portunus trituberculatus (Pt-CHH) consists of four exons and three introns spanning 3849bp in size and generating two mature mRNA, Pt-CHH1, and Pt-CHH2. The primary gene transcript produces a cDNA encoding for the putative Pt-CHH2 from exons 1, 2, 3, and 4 and an alternative transcript encodes for a putative Pt-CHH1 peptide from exons 1, 2, and 4. A promoter fragment of about 3kb was obtained by genomic walking. The tissue-specific expression pattern is examined by reverse transcriptase chain reaction, and the results show that Pt-CHH1 is detected in the eyestalk, brain, muscle, and blood. However, Pt-CHH2 is detected in the ganglia thoracalis and gill. The results indicate that the expression of Pt-CHH2 in the gill might suggest a potential role in osmoregulation. The Pt-CHH transcript level in the gill increases when the crab is exposed to low salinity. The injection of dsRNA for Pt-CHH causes a significant reduction in Pt-CHH2 transcript level and the activity of Na+/K+-ATPase, and carbonic anhydrase (CA) show a serious decrease. In conclusion, this study provides molecular evidence to support the osmoregulatory function of Pt-CHH2.
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