Journal
CELL AND TISSUE BANKING
Volume 20, Issue 2, Pages 173-182Publisher
SPRINGER
DOI: 10.1007/s10561-019-09751-0
Keywords
Mouse; Rat; Calvaria; Osteoblast; Comparative
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Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation and purification of primary osteoblast by enzyme sequential digestion and differentialadhesion. In addition, we compared the phenotypic and functional traits of osteoblasts from mouse and rat which are commonly employed in studies. The method applied equally to rat and mouse in osteoblasts isolation and was corroborated its feasibility and validity. The results also provided us evidences for other experiments such as choosing a certain time point to give intervention and do the relevant tests.
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