Journal
CELL
Volume 176, Issue 3, Pages 419-434Publisher
CELL PRESS
DOI: 10.1016/j.cell.2018.12.035
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Funding
- Max Planck Society
- European Research Countil [725836]
- Bundesministerium fur Bildung und Forschung (BMBF) [01ED1601A, 01EK1606C]
- EU Joint Progamme - Neurodegenerative Disease Research (JPND) (CureALS)
- Human Frontier Science Program [RGP0034/2017]
- Volkswagen Foundation
- NIH [R01GM112846, GM R01-GM081506]
- St. Jude Children's Research Hospital
- American Lebanese Syrian Associated Charities
- HHMI Faculty Scholars program
- European Research Council (ERC) [725836] Funding Source: European Research Council (ERC)
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Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related disease. There is great interest in understanding the underlying biophysical principles and the specific properties of biological condensates with the goal of bringing insights into a wide range of biological processes and systems. The explosion of physiological and pathological contexts involving LLPS requires clear standards for their study. Here, we propose guidelines for rigorous experimental characterization of LLPS processes in vitro and in cells, discuss the caveats of common experimental approaches, and point out experimental and theoretical gaps in the field.
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