Journal
CARBOHYDRATE POLYMERS
Volume 205, Issue -, Pages 176-183Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.09.088
Keywords
Xylooligosaccharides; Bacillus subtilis; Prebiotic; Xylan; Xylanase
Categories
Funding
- Portuguese Foundation for Science and Technology (FCT) [PD/BD/105870/2014, SFRH/BPD/88584/2012, SFRH/BPD/101181/2014, SFRH/BPD/70589/2010, SFRH/BSAB/142873/2018]
- FCT [UID/BIO/04469/2013, POCI-01-0145-FEDER-006684]
- QOPNA research Unit [FCT UID/QUI/00062/2013]
- FEDER [POCI-01-0145-FEDER-016403, POCI-01-0145-FEDER-029549, POCI-01-0145-FEDER-029773, NORTE-01-0145-FEDER-000004]
- European Regional Development Fund [NORTE-01-0145-FEDER-000004]
- Fundação para a Ciência e a Tecnologia [SFRH/BSAB/142873/2018, UID/QUI/00062/2013, PD/BD/105870/2014] Funding Source: FCT
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The global demand of prebiotics such as xylooligosaccharides (XOS) has been growing over the years, motivating the search for different production processes with increased efficiency. In this study, a cloned Bacillus subtilis 3610, containing the xylanase gene xyn2 of Trichoderma reesei coupled with an endogenous secretion tag, was selected for XOS production through direct fermentation of beechwood xylan. A mixture of XOS with a degree of polymerization ranging from 4 to 6 was obtained, presenting high stability after a static in vitro digestion (98.5 +/- 0.2%). The maximum production yield expressed as total XOS per amount of xylan (306 +/- 4 mg/g) was achieved after 8 h of fermentation operating under one-time impulse fed-batch. The optimal conditions found were pH 6.0 and 42.5 degrees C, using 2.5 g/L of initial concentration of xylan increased up to 5.0 g/L at 3 h. Xylopentaose was the major oligosaccharide produced, representing 47% of the total production yield.
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