Journal
CARBOHYDRATE POLYMERS
Volume 208, Issue -, Pages 22-31Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.12.035
Keywords
Chrysanthemum indicum polysaccharide; Duck hepatitis A virus; Autophagosomes; PI3KC3
Categories
Funding
- National Natural Science Foundation of China [31572557, 31772784]
- Special Fund for Agri-scientific Research in the Public Interest [201403051]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
Ask authors/readers for more resources
We previously reported that Chrysanthemum indicum polysaccharide (CIPS) effectively inhibited the replication of duck hepatitis A virus (DHAV). However, the inhibition mechanisms are still unclear. Autophagy plays important role in virus genomic replication. Therefore, in present study, the effect of autophagy on DHAV genome replication as well as the influence of CIPS on autophagy were studied. qPCR, western blot, and ELISA methods were applied to observe the autophagy and analyze the inhibition mechanisms of CIPS on DHAV. Results showed that DHAV infection increased the expression level of LC3-II and interdicted the degradation of p62. Treating with rapamycin benefited DHAV gene expression level. What's more, DHAV infection and rapamycin treatment also promoted the expression of PI3KC3 and increased the concentration of PI3P. However, CIPS treatment significantly downregulated the expressions of LC3-II and PI3KC3 induced by DHAV and rapamycin, and consequently inhibited autophagosomes formation. As a result, DHAV replication was inhibited.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available