Journal
BLOOD
Volume 133, Issue 25, Pages 2669-2681Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2018-09-874578
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Funding
- FRS-FNRS fellowship
- Televie fellowship
- Ludwig Institute for Cancer Research
- Fondation contre le cancer, Salus Sanguinis
- Fondation Les avions de Sebastien [16/21-073]
- Walloon Excellence in Life Sciences and Biotechnology [F 44/8/5-MCF/UIG-10955]
- Ligue Nationale Contre le Cancer
- Institut National du Cancer
- Institut National de la Santeet de la Recherche Medicale (Inserm)
- de Duve Institute Morange Funds postdoctoral fellowship
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Calreticulin (CALR) +1 frameshift mutations in exon 9 are prevalent in myeloproliferative neoplasms. Mutant CALRs possess a new C-terminal sequence rich in positively charged amino acids, leading to activation of the thrombopoietin receptor (TpoR/MPL). We show that the new sequence endows the mutant CALR with rogue chaperone activity, stabilizing a dimeric state and transporting TpoR and mutants thereof to the cell surface in states that would not pass quality control; this function is absolutely required for oncogenic transformation. Mutant CALRs determine traffic via the secretory pathway of partially immature TpoR, as they protect N117-linked glycans from further processing in the Golgi apparatus. A number of engineered or disease-associated TpoRs such as TpoR/MPL R102P, which causes congenital thrombocytopenia, are rescued for traffic and function by mutant CALRs, which can also overcome endoplasmic reticulum retention signals on TpoR. In addition to requiring N-glycosylation of TpoR, mutant CALRs require a hydrophobic patch located in the extracellular domain of TpoR to induce TpoR thermal stability and initial intracellular activation, whereas full activation requires cell surface localization of TpoR. Thus, mutant CALRs are rogue chaperones for TpoR and traffic-defective TpoR mutants, a function required for the oncogenic effects.
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