Journal
BLOOD
Volume 133, Issue 12, Pages 1308-1312Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2018-11-885368
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Funding
- National Institutes of Health, National Heart, Lung, and Blood Institute T32 training grant in molecular hematology [HL007088]
- Philip Majerus Fellowship Fund
- National Science Foundation
- Howard Hughes Medical Institute
- National Institutes of Health, National Institute of General Medical Sciences [R01GM45443]
- American Society of Hematology Scholar Award
- National Institutes of Health, National Heart, Lung, and Blood Institute [4R00HL114732, 1R01HL137793]
- V Foundation for Cancer Research
- Edward Mallinckrodt Jr Foundation
- AA&MDS International Foundation
- CONCERN Foundation
- American Federation for Aging Research
- Longer Life Foundation
- Center for Regenerative Medicine at Washington University in St. Louis
- Department of Defense Bone Marrow Failure Research Program [BM160054]
- CDMRP [BM160054, 917355] Funding Source: Federal RePORTER
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Reduced levels of TERC, the telomerase RNA component, cause dyskeratosis congenita (DC) in patients harboring mutations in TERC, PARN, NOP10, NHP2, NAF1, or DKC1. Inhibition of the noncanonical poly(A) polymerase PAPD5, or the exosome RNA degradation complex, partially restores TERC levels in immortalized DKC1 mutant cells, but it remains unknown if modulation of posttranscriptional processing of TERC could improve hematopoietic output in DC. We used human embryonic stem cells (hESCs) with a common dyskerin mutation (DKC1_A353V), which have defective telomere maintenance and reduced definitive hematopoietic potential, to understand the effects of reducing EXOSC3 activity, or silencing PAPD5-mediated oligoadenylation, on hematopoietic progenitor specification and function in DC. Reduction of EXOSC3 or PAPD5 levels in DKC1 mutant hESCs led to functional improvements in TERC levels and telomerase activity, with concomitant telomere elongation and reduced levels of DNA damage signaling. Interestingly, the silencing of PAPD5, but not EXOSC3, significantly restored definitive hematopoietic potential in DKC1 mutant cells. Mechanistically, we show that PAPD5 inhibition is sustained in differentiated CD34(+) cells, with a concomitant increase in mature, functional, forms of TERC, indicating that regulation of PAPD5 is a potential strategy to reverse hematologic dysfunction in DC patients.
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