Journal
BIOSENSORS & BIOELECTRONICS
Volume 134, Issue -, Pages 8-15Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.03.043
Keywords
ZrHf-based metal-organic framework; Electrochemical aptasensor; Early diagnosis of HER2; Detection of MCF-7 cells
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Funding
- National Natural Science Foundation of China [U1604127, U1704256, 81601082]
- Innovative Technology Team of Henan Province [CXTD2014042]
- Key Research Project of University of Henan Province [19zx004]
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We report here a new bimetallic ZrHf metal-organic framework (ZrHf-MOF) embedded with abundant carbon dots (CDs) (denoted as CDs@ZrHf-MOF), which exhibits strong fluorescence and rich-amino-functionalization. The CDs@ZrHf-MOF can be applied as the scaffold for anchoring aptamer strands to determine human epidermal growth factor receptor-2 (HER2) and living HER2-overexpressed MCF-7 cells. The basic characterizations reveal that the CDs are embedded within the interior cavities of ZrHf-MOF without varying the nanostructure, leading to good biocompatibility, strong fluorescence, and high electrochemical activity of CDs@ZrHf-MOF. As compared with the pristine ZrHf-MOF, the CDs@ZrHf-MOF-based electrochemical aptasensor displays better sensing performances toward both HER-2 and MCF-7 cells, giving an extremely low detection limit of 19 fg mL(-1) (HER2 concentration range: 0.001-10 ng mL(-1)) and 23 cell (cell concentration range: 1 x 10(2)similar to 1 x 10(5) cell mL(-1)), with good selectivity, stability, reproducibility, and acceptable applicability. The proposed strategy for developing CDs@ZrHf-MOF-based aptasensor is promising for the early and sensitive detection of cancer markers and living cancer cells.
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