Journal
BIOSENSORS & BIOELECTRONICS
Volume 128, Issue -, Pages 76-82Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.11.045
Keywords
Fe3O4; Polypyrrole; E-DNA sensor; PAMAM; Mycobacterium tuberculosis; Naphthoquinone
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Funding
- Ministry of Higher Education and Scientific Tunisia
- University of Tunis El Manar Tunisia
- Tunisian Ministry of Higher Education and Scientific Research of Tunisia through PRF Project [39382RE]
- Ministry of European Affairs and Foreign Affairs (MEAE) through PHC-Maghreb [39382RE]
- Ministry of Foreign Affairs, Research and Innovation (MESRI) from France through PHC-Maghreb [39382RE]
- Tunisian Ministry of Higher Education and Scientific Research of Tunisia through PHC-Maghreb [39382RE]
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In this work, we achieved the selective detection of wild and mutated rpoB gene in M. tuberculosis using an electrochemical DNA (E-DNA) sensor based on polypyrrole/Fe3O4 nanocomposite bearing redox naphthoquinone tag on PAMAM (spaNQ/PAMAM/PPy/Fe3O4). The hybridization between a given probe and the complementary DNA target induced a large decrease in the naphthoquinone redox signal as measured by SWV and no cross-hybridization with single nucleotide mismatch DNA target occurred. Thanks to the catalytic properties of iron oxide nanoparticles combined with conducting properties of polypyrrole platform, we demonstrated that the transducing system allowed the detection of 1 fM of DNA target in a 50-mu L drop corresponding to 3 x 10(4) copies of DNA. The sensor was able to detect the rpoB gene in PCR-amplified samples of genomic DNA and could also discriminate between the wild type rpoB gene and a single nucleotide mutated rpoB gene that provides resistance to rifampicin. Furthermore, the sensor could selectively detect the wild and mutant DNA in genomic samples without PCR amplification.
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