Journal
BIOSENSORS & BIOELECTRONICS
Volume 128, Issue -, Pages 122-128Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.12.032
Keywords
Retinol binding protein 4; Self-assembled monolayer; Impedance spectroscopy; Picomolar detection
Categories
Funding
- CSIR, India [31/28(216)]
- EMBO [ASTF 564-2016]
- [MLP-0018]
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Type (II) diabetes is one of the major threats to mankind as it causes insulin resistance in human body and Retinol Binding Protein 4 (RBP4) is currently considered as a potential biomarker for early management of this disease. Hence a low-level detection of RBP4 is a very important task and for this purpose, a novel RBP4 biosensor has been developed using homemade plastic chip electrodes (PCEs) as a platform for self-assembled monolayer (SAM) of 4-ATP and further functionalization with glutaraldehyde. Anti RBP4 is used as biorecognition species and electrochemical impedance spectroscopy has been performed to detect different RBP4 concentrations plotted against charge transfer resistance. A wide concentration range from 100 fg/mL to 1 ng/mL has been tested and a low limit of detection (LOD) of 100 fg/mL has been achieved. This is the first report for fabrication of electrochemical biosensor of RBP4 using Ag-Ab interaction having such low LOD. The sensor is characterized by various physico-chemical techniques. Excellent reproducibility and quick measurement make this biosensor extremely useful for the biomedical industry.
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