Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 83, Issue 5, Pages 824-828Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2019.1574204
Keywords
Fluorescent reporter; ATP; ADP; yeast; diauxic shift
Categories
Funding
- Ohsumi Frontier Science Foundation
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PercevalHR (Perceval High Resolution) is an artificially designed fluorescent protein, which changes its excitation spectrum based on the ADP/ATP ratio of the environment. Here we demonstrated that PercevalHR can be used for monitoring energy status of Saccharomyces cerevisiae cells, which are affected by diauxic shift and mitochondria inhibition, in a non-invasive and non-destructive manner.
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