Journal
BIORESOURCE TECHNOLOGY
Volume 273, Issue -, Pages 1-7Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.10.037
Keywords
Cellulose; Ball milling; Cellulase adsorption; Physicochemical properties; Enzymatic hydrolysis
Funding
- National Natural Science Foundation of China [31671572]
- National Key R&D Program of China [2016YFE0112800]
- Program for Changjiang Scholars and Innovative Research Team of China Ministry of Education [IRT-17R105]
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This study evaluated the effects of physicochemical properties of a series of ball-milled cellulose on cellulase adsorption and glucose yield. The relationship between cellulase adsorption and initial hydrolysis rate was also discussed. We found that hydrophobicity and surface charge are the key factors affecting cellulase adsorption on ball-milled cellulose. The results demonstrated that glucose yield had a positive correlation with specific surface area, while showed a negative correlation with particle size, degree of polymerization and crystallinity. Among these properties, specific surface area and crystallinity are the key factors affecting glucose yield. As ball milling progressed, cellulose showed lower enzyme adsorption capacity/amount of bound enzyme during initial stage of hydrolysis, but had higher initial hydrolysis rate. The enhanced rate is attributed to the fact that the amorphous region produced by ball milling reduces the free energy required for decrystallization thus increases the catalytic efficiency of the bound enzyme.
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