Journal
BIORESOURCE TECHNOLOGY
Volume 274, Issue -, Pages 468-478Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.12.017
Keywords
Urban wastewater treatment plant; Constructed wetland; Antibiotic resistance; Enterococcus faecium; Enterococcus faecalis; Clones
Funding
- EU (FP7 grant) [311933]
- SIMTREA at DeFENS, University of Milan
- University of Catania
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Enterococci isolated from different sites of an urban wastewater treatment plant (consisting of three horizontal subsurface flow constructed wetlands) were investigated. One-hundred-thirty isolates were identified at species level and tested for resistance to eleven antibiotics, by microdilution method, and their clonal relatedness was established by SmaI-PFGE analysis. Results highlighted the persistence of enterococcal population in all effluents and the dominance of E. faecium species. A high incidence of antibiotic resistance against erythromycin, chloramphenicol, rifampicin and ampicillin was observed, with 120 strains (93%) showing a multi-drug-resistance. Numerous pulso-types with a unique pattern were detected indicating a high diversity within enterococcal population. The recurrence of some pulso-types in different effluents was disclosed and, within the same pulso-types, different resistance patterns were observed. Comparing the MIC values of strains from inlet and outlet, different trends were observed, highlighting a certain variability among constructed wetlands in affecting the antibiotic resistance among enterococcal population.
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