Journal
BIORESOURCE TECHNOLOGY
Volume 274, Issue -, Pages 120-126Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.11.082
Keywords
Haematococcus pluvialis cyst; Ionic liquid; Astaxanthin extraction; Lipid extraction; Cell disruption
Funding
- Advanced Biomass R&D Center (ABC) of the Global Frontier Project - Ministry of Science and ICT, Republic of Korea [ABC- 2012M3A6A205388]
- Korea Institute of Energy Research (KIER) [B8-2432-04]
- Korea CCS R&D Center (Korea CCS 2020 Project) - Korea government (Ministry of Science and ICT) [KCRC-2014M1A8A1049278]
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Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)(2)N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 degrees C, 60 min), almost complete astaxanthin recoveries (> 99%) along with moderate lipid extractions (similar to 82%) could be obtained.
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