Journal
BIOCHEMISTRY
Volume 58, Issue 14, Pages 1841-1844Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.9b00110
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Funding
- National Science Foundation [CHE-1465096, S10 RR027273]
- NIH-NIGMS [P20 GM103446]
- NSF [IIA-13017455]
- State of Delaware
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We demonstrate that time-resolved second harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport of the amphiphilic ion, malachite green, across the plasma membrane in living human dermal fibroblast cells. Analysis of the time-resolved SH images reveals that membrane regions, which appear to be enduring higher stress, exhibit slower transport rates. It is proposed that this stress-transport relation may be a result of local enrichment of membrane rigidifiers as part of a response to maintain membrane integrity under strain.
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