Journal
BIOCHEMISTRY
Volume 58, Issue 9, Pages 1181-1183Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.9b00012
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Funding
- Robert A. Welch Foundation [A0034]
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HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the HcbA1-catalyzed reaction. Detailed mechanistic studies provide evidence for nucleophilic aromatic substitution and flavin-N5-oxide formation.
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