Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 143, Issue -, Pages 169-178Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2018.12.019
Keywords
2,3-Butanediol; Enterobacter aerogenes; Biorefinery; Metabolic engineering
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2014R1A2A2A01007321]
- Ministry of Education [2016R1D1A1A09918327]
Ask authors/readers for more resources
The enhanced production of 2,3-butanediol was investigated using a metabolic engineering approach and optimized fermentation conditions. New engineered strains of Enterobacter aerogenes ATCC 29007 were developed by deleting the D-lactate dehydrogenase (ldhA), phosphate acetyltransferase (pta), malate dehydrogenase (mdh), and acetaldehyde dehydrogenase (acdh) genes to block the production of lactate, acetate, succinate, and ethanol, respectively. The resulting engineered strain E. aerogenes SUMI02 (Delta ldhA Delta pta) produced 36.5 g/L of 2,3-butanediol in flask cultivation, an amount 8.11 times greater than that of its wild type counterpart (4.5 g/L). In addition, the 2,3-butanediol production and productivity reached 38.24 g/L and 0.8 g/L/h, respectively, in the batch fermentation using a bioreactor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available