Journal
AUTOPHAGY
Volume 15, Issue 5, Pages 922-923Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2019.1582952
Keywords
Autophagosome formation; CRISPR; DedA family; GFP-LC3-RFP reporter; lipid droplet; VMP1; VTT domain
Categories
Funding
- Japan Science and Technology Agency [JPMJER1702]
Ask authors/readers for more resources
Macroautophagy/autophagy requires many autophagy-related (ATG) proteins. Most of the ATG genes were identified by genetic screening using simple model organisms. Recently, we performed a forward genetic screen in mammalian cells using the CRISPR-Cas9 system and our autophagic flux reporter GFP-LC3-RFP. One of the identified proteins was TMEM41B, an ER-localized multi-spanning membrane protein. TMEM41B has a characteristic transmembrane domain (the VTT domain), which is also found in VMP1, another protein involved in autophagy. Our results show that TMEM41B and VMP1 are physically and functionally associated.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available