Journal
ARCHIVES OF VIROLOGY
Volume 164, Issue 5, Pages 1453-1457Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-019-04207-y
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Funding
- Cornell Latin American Studies Program Fellowship
- USDA-NIFA [1013513]
- College of Agriculture and Life Sciences, Cornell University
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Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.
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