Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 662, Issue -, Pages 83-92Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2018.12.001
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Funding
- JSPS-Kakenhi from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [24108001, 24108002, 17H02850]
- Grants-in-Aid for Scientific Research [17H02850] Funding Source: KAKEN
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Non-equilibrium atmospheric pressure plasma (NEAPP) is a mixture of radicals, electrons, anions, cations and light at near body temperature. Plasma-activated medium (PAM) is realized using NEAPP provided by engineered devices and irradiated to a cell culture medium for a period of 600 s. Glioblastoma cells U251SP cultivated in PAM previously indicated that antitumor effects induced PAM-specific apoptotic cell-death. Metabolomic profiles of a hundred intracellular metabolites were analyzed using capillary electrophoresis mass spectrometry. The metabolomic profiles of the PAM-treated U251SP cells were changed significantly with inhibition of the glycolysis pathway and with enhancement of the pentose phosphate pathway.
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