Journal
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 88
Volume 88, Issue -, Pages 725-783Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev-biochem-062917-011901
Keywords
nuclear pore complex; nucleocytoplasmic transport; mRNA export; integrative structural biology; X-ray crystallography; electron microscopy
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM111461, R01GM117360] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM111461, R01 GM117360] Funding Source: Medline
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The nuclear pore complex (NPC) serves as the sole bidirectional gateway of macromolecules in and out of the nucleus. Owing to its size and complexity (similar to 1,000 protein subunits, similar to 110 MDa in humans), the NPC has remained one of the foremost challenges for structure determination. Structural studies have now provided atomic-resolution crystal structures of most nucleoporins. The acquisition of these structures, combined with biochemical reconstitution experiments, cross-linking mass spectrometry, and cryoelectron tomography, has facilitated the determination of the near-atomic overall architecture of the symmetric core of the human, fungal, and algal NPCs. Here, we discuss the insights gained from these new advances and outstanding issues regarding NPC structure and function. The powerful combination of bottom-up and top-down approaches toward determining the structure of the NPC offers a paradigm for uncovering the architectures of other complex biological machines to near-atomic resolution.
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