Journal
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 12
Volume 12, Issue -, Pages 129-150Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev-anchem-061318-115027
Keywords
bioluminescence; fluorescence; chemiluminescence; genetically encoded biosensor; in vivo imaging; luciferin; luciferase; bioluminescence resonance energy transfer; BRET
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Funding
- NIGMS NIH HHS [R01 GM118675, R01 GM129291] Funding Source: Medline
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Although fluorescent reporters and biosensors have become indispensable tools in biological and biomedical fields, fluorescence measurements require external excitation light, thereby limiting their use in thick tissues and live animals. Bioluminescent reporters and biosensors may potentially overcome this hurdle because they use enzyme-catalyzed exothermic biochemical reactions to generate excited-state emitters. This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors. Lastly, we discuss chemiluminescent sensors that produce photons in the absence of luciferases. This review aims to explore fundamentals and experimental insights and to emphasize the yet-to-be-reached potential of next-generation luminescent reporters and biosensors.
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