Journal
ANNALS OF APPLIED BIOLOGY
Volume 174, Issue 3, Pages 284-292Publisher
WILEY
DOI: 10.1111/aab.12491
Keywords
geographical distribution; mixed genotype infections; PepMV; population structure; tomato crop; viral genetic diversity
Categories
Funding
- Ministry of Economy, Industry and Competitiveness [PCIN-2017-055]
- Ministere de l'Enseignement Superieur, de la Recherche Scientifique de la Formation des Cadres [AGL2014-59556-R, FPU16/02569, PTQ-13-05882, PTQ-15-07646]
Ask authors/readers for more resources
Pepino mosaic virus (PepMV, genus Potexvirus) is an emergent and highly infectious pathogen responsible for economically important diseases in tomato crops. An extensive survey of tomato plants showing PepMV-like symptoms was carried out in 2017 to study the PepMV genetic diversity and populations structure in different tomato-producing areas of Spain and Morocco. Molecular dot-blot hybridization analysis showed that virus populations from Spain and Morocco were mainly composed of isolates belonging to the Chilean 2 (CH2) strain, although isolates of the European (EU) strain were detected in significant proportions in Spanish populations, mainly in mixed infections. A few isolates of the American (US1) strain were also detected in Tenerife (Canary Islands, Spain) crops. Eighty-five isolates were randomly selected and sequenced in the genomic region that encodes the triple gene block and capsid protein genes. Our phylogenetic and population genetics analyses confirmed the presence of the CH2, EU and US1 PepMV strains. Despite the high genetic similarity observed within populations, variants were maintained at low frequency under purifying selection, and differentiation among more geographically distant locations was identified, with potential gene flow contributing to the shaping of the PepMV populations structure.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available