Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 19, Pages 6376-6379Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201902664
Keywords
biosensors; diabetes monitoring; dual-marker detection; electrochemistry; immunoassays
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Funding
- LeonaM. and HarryB. Helmsley Charitable Trust [2018PG-TI0061]
- Turkish Scientific and Technological Research Council (TUBITAK)
- Spanish Ministry of Economy
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Performing bioassay formats based on enzyme and antibody recognition reactions with a single detection chip remains an unmet challenge owing to the different requirements of such bioassays. Herein, we describe a dual-marker biosensor chip, integrating enzyme and antibody-based assays for simultaneous electrochemical measurements of insulin (I) and glucose (G). Simultaneous G/I sensing has been realized by addressing key fabrication and operational challenges associated with the different assay requirements and surface chemistry. The I immunosensor relies on a peroxidase-labeled sandwich immunoassay, while G is monitored through reaction with glucose oxidase. The dual diabetes biomarker chip offers selective and reproducible detection of picomolar I and millimolar G concentrations in a single microliter sample droplet within less than 30min, including direct measurements in whole blood and saliva samples. The resulting integrated enzymatic-immunoassay biosensor chip opens a new realm in point-of-care multiplexed biomarker detection.
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