Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 6, Pages 3893-3901Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b04634
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [31770093, 91751207, 21477062]
- Natural Science Foundation of Shandong Province [ZR2016CM03, ZR2017ZB0210]
Ask authors/readers for more resources
Polysulfides are newly discovered cellular contents, and they are involved in multiple intracellular processes, including redox homeostasis and protein sulfhydration. The dynamic changes of polysulfides inside the cell are directly related to these processes. To monitor the intracellular dynamics and subcellular levels of polysulfides, we developed green-fluorescent-protein (GFP)-based probes that are polysulfide-specific. A pair of cysteine residues was introduced near the GFP chromophore with the spatial distance between the cysteine residues designed to allow the formation of internal -S-n- (n >= 3) bonds but not -S-2- (disulfide) bonds. We tested these probes in model microorganisms and found that they displayed ratiometric changes to intracellular polysulfides that had clear variations associated with the growth phases. The distribution of polysulfides in subcellular organelles is heterogeneous, suggesting that polysulfides have multiple origins and functions in cells. These probes provided long-desired tools for polysulfide in vivo studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available