Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 7, Pages 4520-4528Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b05440
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [Sp314/13-1, INST 162/500-1 FUGG]
- State of Hesse, LOEWE Center DRUID
- Promotionskolleg Bioressourcen and Biotechnologie of the Technische Hochschule Mittelhessen
- Justus Liebig University Giessen
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Schistosomes are parasitic platyhelminthes that cause schistosomiasis, which is a life-threatening infectious disease for humans in the tropics and subtropics worldwide. Within the human host, female and male schistosomes develop and pair as a prerequisite for egg production. Part of the eggs get lodged in organs such as the gut, spleen, and liver, where they cause severe inflammatory processes, including liver fibrosis, which is one of the most serious pathological symptoms. High-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) mass spectrometry imaging (MSI) has been used as a powerful tool to investigate adult schistosomes at the topographic molecular level. An MSI-compatible protocol was developed, covering critical sample preparation steps and focusing on obtaining artifact-free, longitudinal cryosections. Planar, consecutive sections were prepared from similar to 400 mu m thick S. mansoni worm couples, comparing several microembedding approaches. High-resolution MSI at both, 10 and 5 mu m lateral resolution unraveled anatomical structures and differential abundances of glycerophospholipids and saccharides in females and males. In addition, glycerophospholipids occurred differentially abundant in worm tissues of the female, such as the gut, which is essential for nutrient uptake and subsequent metabolism. Fragment ions of isobaric phospholipids were investigated by on-tissue MS2 imaging experiments, unambiguously showing isomer-specific ion signals. This study provides a solid basis for investigating schistosome parasites in chemical detail at the whole-worm level by MSI.
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