Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 5, Pages 3533-3538Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b05268
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Funding
- Department of Science and Technology (Government of India) [YSS/2015/001712, DST 11-IFA-PH-07]
- IIT Madras
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Strategies for simultaneous detection and detoxification of Hg2+ using a single sensor from biological and environmental samples are limited and have not been realized in living organisms so far. We report a highly selective, small molecule turn-on fluorescent sensor, PYDMSA, based on the cationic dye Pyronin Y (PY) and chelating agent meso-2,3-dimercaptosuccinic acid (DMSA) for the simultaneous detection and detoxification of inorganic mercury (Hg2+). After Hg2+ detection, concomitant detoxification was carried out with sufficient efficacy in living samples, which makes the sensor unique. PYDMSA exhibits high selectivity for Hg2+ over other competing metal ions with an experimental detection limit of similar to 300 mu M in aqueous buffer solution. When PYDMSA reacts with Hg2+, the CS-C-9 bond in the sensor gets cleaved. This results in the turn-on response of the fluorescence probe with a concomitant release of one equivalent of water-soluble Hg2+ DMSA complex which leads to a synchronous detoxifying effect. The sensor by itself is nontoxic to cells in culture and has been used to monitor the real-time uptake of Hg2+ in live cells and zebrafish larvae. Thus, PYDMSA is a unique sensor which can be used to detect and detoxify mercury at the same time in living samples.
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