Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 411, Issue 10, Pages 2169-2175Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-019-01652-1
Keywords
Lateral flow assay; Fluorescent quenching; Zearalenone; Quantum dots
Funding
- National Key Technology R D Program [2008BAK42B05]
- Guangdong Province Key Scientific Research [2013A022100031]
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Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78-25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1-93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.
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