Journal
ANALYTICA CHIMICA ACTA
Volume 1064, Issue -, Pages 33-39Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2019.02.060
Keywords
Electrochemical biosensor; MicroRNAs; Duplex specific nuclease; Horseradish peroxidase; Signal amplification
Categories
Funding
- National Natural Science Foundation of China [21375063, 21575069]
- Natural Science Foundation of Jiangsu Province [BK20181382]
- Jiangsu Collaborative
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The detection of sequence-specific microRNAs (miRNAs) is an important factor to the diseases diagnosis. Herein, a triple signal amplification electrochemical biosensor for highly sensitive detection of miRNA-21 was developed based on a duplex-specific nuclease (DSN)-assisted target recycling combined with gold nanoparticles (NPs), horseradish peroxidase (HRP) enzymatic signal amplification. The electrochemical biosensor generated significantly amplified amperometric current changes (Delta i) for the detection of miRNA-21 down to 43.3 aM, and Delta iwas proportional to the logarithm of the concentration of miRNA-21 within the range of 0.1 fM to 100 pM. Meanwhile, the inherent selectivity of the term hairpin capture probe endowed the biosensor with high differentiation of similar miRNAs. The good feasibility of the proposed strategy for cell miRNA detection was confirmed by analyzing miRNA-21 in A549 lysates, which indicates its promising potential in biomedical research and clinical analysis. (C) 2019 Published by Elsevier B.V.
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