Journal
ANALYTICA CHIMICA ACTA
Volume 1051, Issue -, Pages 103-109Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.11.001
Keywords
Gas chromatography; Quadrupole time-of-flight mass spectrometry; Atmospheric pressure chemical ionization source; Very long-chain polyunsaturated fatty acids
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Funding
- Programa Estatal de Investigacion, Desarrollo e Innovacion orientada a los retos de la sociedad (Ministry of Economy and Competitiveness): Research Project Acidos grasos poliinsaturados de cadena muy larga en peces: biosintesis e implicaciones durante fas [AGL2013-40986-R]
- Ministry of Economy and Competitiveness [IJCI-2014-20588]
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Gas chromatography coupled to a quadrupole time-of-flight mass analyzer (QTOF) with an atmospheric pressure chemical ionization (APCI) source has been tested to study the ionization and mass spectrometric behavior of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFAs, C18-24; VLC-PUFAs, >C-24). The protonated molecule ([M+H](+)), measured at accurate mass, became the base peak of the spectrum for all the studied compounds and was promoted by the addition of water into the source. This fact overcame the existing difficulties for the identification of VLC-PUFAs when using an electron ionization source (EI). The extensive fragmentation of PUFAs in this source is the main drawback due to the fact that since reference standards are not commercially available, final identification relies on retention time estimation. The application of GC-APCI-QTOF to the screening of lipid extracts from the eyes of different fish species added confidence to the identification of several VLC-PUFAs. Further investigation of ion ratios allowed to predict the position of key double bonds enabling the classification of VLC-PUFAs as omega 3 or omega 6 compounds. VLC-PUFAs spectra found in biological samples were compared to those obtained from corresponding peaks found in heterologous expression experiments of fish's Elovl4. (C) 2018 Elsevier B.V. All rights reserved.
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