Journal
ANALYTICA CHIMICA ACTA
Volume 1048, Issue -, Pages 154-160Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.10.020
Keywords
Food safety; Listeria monocytogenes; Gold nanorods; Multicolorimetric detection; Immunomagnetic separation; Artificial enzyme
Categories
Funding
- Chinese National Natural Science Foundation [81602894, 81602895]
- China Postdoctoral Science Foundation [2017T100214, 2016M591492]
- Development Foundation of Science and Technology in Jilin Province of China [20170204003SF, 20180520132JH]
- Development of Education in Jilin Province of China [JJKH20170873KJ, JJKH20180240KJ]
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Listeria monocytogenes (L. monocytogenes) is one of the most common food-borne pathogens. The authors describe a sensitive and reliable multicolorimetric assay for L. monocytogenes using a sensing system based on TMB2+ etching of gold nanorods. Apt-MNP was used as the capture probe, and IgY-BSA-MnO2 NPs was chosen as an oxidase-like nano-artificial enzyme to oxidize TMB to generate TMB2+. Under the optimized conditions, the longitudinal shift of localized surface plasmon resonances had a linear correlation with the L. monocytogenes concentration in the range between 10 to 10(6) cfu mL(-1). Meanwhile, the sensing system can generate vivid color responses as colorful as a rainbow, and the limit of detection is as low as 10 cfu mL(-1) at a glance. Recoveries ranging from 97.4 to 101.3% are found when analyzing spiked food samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of L. monocytogenes. (C) 2018 Elsevier B.V. All rights reserved.
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